General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS CXCR3 β-Arrestin Cell Line, 2 vials. (93-0271C3)
The PathHunter CXCR3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR3 and EA-tagged β-Arrestin-2. Activation of CXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Peptostreptococcus anaerobius; Strain VPI 4330
Peptostreptococcus anaerobius strain VPI 4330 has media testing and quality control applications. This whole-genome sequenced bacterial type strain is propagated anaerobically and requires proline for glucose utilization.
Biochain Institute Inc Frozen Tissue Section - Lupus: Adrenal, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx ChemiScreen CCK2 Cholecystokinin Receptor Membrane Preparation, 1 vial. (HTS083M)
The ChemiScreen CCK2 Cholecystokinin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCKBR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC M059J; Malignant Glioblastoma; Human (Homo sapiens)
M059J is a glial cell isolated from the brain of a 33-year-old, male patient with malignant glioblastoma. These cell lines provide helpful model systems in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair. It can be used in neuroscience research.
Eurofins Discoverx Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells, 2 Vials of Cells. (HTS076RTA)
The Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. PAF) based activation of PTAFR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTAFR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTAFR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Biochain Institute Inc Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Liver, 2x10 ug/PK
BioChain's human DNA matched pair products include: Primary Pair (PP), Primary and Metastasis Pair (PM). PP consists of DNA prepared from primary tumor and its adjacent normal tissue; PM consists of DNA prepared from primary tumor and corresponding ponding metastatic tumor. Genomic DNA in each pair is prepared from the same donor. This product line is designed foridentifying tumor-specific genes and tumor metastatic genes.
Eurofins Discoverx PathHunter U2OS ACVR1C/ACVR2 Dimerization Cell Line, 2 vials. (93-1069C3)
The PathHunter ACVR1C/ACVR2 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. activin B) induced dimerization of ACVR1C/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR1C target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1C/ACVR2, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
